Cell Systems and Imaging (CSI)
Ghent University, Coupure Links 653, 9000 Ghent

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Basic Research

• Cellular basis of aging and premature aging disease; fundamental understanding of cellular deregulation in laminopathies.
• Expertise: cell-based assays, genetic engineering, advanced light microscopy, expression profiling.

Equipment and facilities

• Advanced light microscopy (widefield, confocal, multiphoton, Raman) and image analysis, high content cytometry, live cell imaging, cell culture (L2 and L3), genomics (qPCR, microarray, bioinformatics, etc) and proteomics (MS)

• W. De Vos, R. Hoebe, G. Joss, W. Haffmans, S. Baatout, P. Van Oostveldt and E. Manders (2009). Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle. Cytometry Part A 75(5), p.428-439.
• W. De Vos, L. Van Neste, B. Dieriks, G. Joss and P. Van Oostveldt (2010). High content image cytometry in the context of subnuclear organisation. Cytometry Part A 77(1), p.64-75.
• W. De Vos, F. Houben, R. A. Hoebe, R. Hennekam, B. Van Engelen, E. Manders, F. Ramaekers, J. Broers and P. Van Oostveldt (2010). Increased plasticity of the nuclear envelope and hypermobility of telomeres due to the loss of A-type lamins. Biochimica et Biophysica Acta – General Subjects 1800(4), p.448-458.
• J. De Vylder, W. De Vos, E. Manders and W. Philips (2011). 2D mapping of strongly deformable cell nuclei based on contour matching. Cytometry Part A, 79(7), p.580-588.  
• W. De Vos, F. Houben, M. Kamps, J. Cox, P. Van Oostveldt, V. Verstraeten, M. van Steensel, C. Marcelis, A. van den Wijngaard, F. Ramaekers and J. Broers (2011). Repetitive Disruptions of the Nuclear Envelope Invoke Temporary Loss of Cellular Compartmentalization in Laminopathies. Human Molecular Genetics 20(21), p.4175-4186. 
• P. Back, W. De Vos, J. Vanfleteren and B. Braeckman (2011). Exploring real-time in vivo redox biology in Caenorhabditis elegans, Free Radical Biology and Medicine 52(5), p.850-859. 
• T. Sieprath, R. Darwiche and W. De Vos (2012). Lamins as mediators of oxidative stress. Biochemical and Biophysical Research Communications 421, p.635-639. 
• F. Houben, W. De Vos, I. Krapels, M. Coorens, G. Kierkels, M. Kamps, V. Verstraeten, C. Marcelis, A. van den Wijngaard, F. Ramaekers and J. Broers (2013). Cytoplasmic localization of PML foci in laminopathies. Histochemistry and Cell Biology, 139(1), p.119-134.