Prof. Thierry Arnould / Prof. Patricia Renard
Unité de Recherche en Biologie Cellulaire
University of Namur, Namur Research Institute for Life Sciences (NARILIS)
61, rue de Bruxelles, 5000 Namur
Phone: +32 (0)81724125
Research Lines
- Analysis of the mitochondrial biogenesis (characterization and role) during the differentiation of different mesenchymal stem cells.
- Adipocyte
cell responses triggered by mitochondrial uncoupling and dysfunction
- Impact and effects of ER stress or lysosomal storage disorder on mitochondrial morphology and activity.
- Relation « host-pathogens » between a eukaryotic cell and a facultative intracellular bacteria
Facilities
- Cell culture facilities and the regular equipment for cell biology and biochemsitry,
- Real-time PCR for microfluidics cards
- Confocal microscope and a BD Pathway 855 Imaging Systems
- A complete proteomic platform for 2D-DIGE analysis and identification of proteins by mass spectrometry (MALDI-TOF and Q-TOF2-LC MS-MS).
- The laboratory also belongs to NARILIS (an Institute for life Sciences) and has access to many facilities
Publications
- Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction.
- Mitochondrial (dys)function in adipocyte (de)differentiation and systemic metabolic alterations.
- Mild mitochondrial uncoupling induces 3T3-L1 adipocyte de-differentiation by a PPARgamma-independent mechanism, whereas TNFalpha-induced de-differentiation is PPARgamma dependent.
- CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes.
- Mitochondrial biogenesis in mtDNA-depleted cells involves a Ca2+-dependent pathway and a reduced mitochondrial protein import.
- Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: role of fatty acid beta-oxidation and glucose.
- CREB activation induced by mitochondrial dysfunction is a new signaling pathway that impairs cell proliferation.